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Potential of mesenchymal stem cells and nanoparticles in the treatment of retinal disorders
Kettner, Ondřej ; Heřmánková, Barbora (advisor) ; Filová, Eva (referee)
Retinal degenerative diseases are recognized worldwide as one of the predominant causes underlying irreversible visual impairment and even blindness. The current standard treatment has helped in both the prevention and supportive treatment of several pathologies, especially if they relate to the retina. However, many of these methods are rather invasive in terms of procedure with the subsequent possibility of infection, inflammation or even retinal detachment with the potential risk of vision loss. These restrictions represent a significant cost to patients' quality of life and also have an economic impact on the healthcare system, so new treatment options are being sought. Mesenchymal stem cells (MSCs) are a promising cell therapy tool for the treatment of many previously untreatable defects and diseases. In addition to MSCs, the use of nanoparticles and nanofibrous carriers is currently being studied in the diagnosis and therapy of retinal diseases. Combining the immunomodulatory and regenerative properties of MSCs and nanotechnologies offers a promising therapeutic strategy for a number of diseases. This thesis is therefore devoted to the current state of the use of nanomaterials, MSCs and their combination that may provide new opportunities in the treatment of retinal degenerative diseases.
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Experimental models of a transfer of stem cells for therapeutic purposes
Faltýsková, Helena ; Krulová, Magdaléna (advisor) ; Indrová, Marie (referee)
Experimental models of a transfer of stem cells for therapeutic purposes Abstract Stem cell therapy currently represents a standard procedure of treating a wide variety of hereditary diseases and serious injuries. Development of the most suitable way of transfer of stem cells into the patient body remains very important question concerning this type of therapy. In our experiments we used nanofiber scaffolds for stem cell cultivation and their subsequent transfer. These nanofibers were prepared by the original needleless electrospun NanospiderTM technology. Allogeneic cornea or skin graft were transplanted from B6 mice to BALB/c mice. The grafts were covered by a nanofibrous scaffold with cultivated stem cells. Stem cells were stained by an imunofluorescent dye to enable us to monitore their migration from nanofibers into tissues and consequent distribution in the body and characterize changes of this distribution in the time. The methods of ELISA and PCR were used to confirm that mesenchymal stem cells support the production of antiinflammatory cytokines IL-4 and IL-10 and contribute to inhibition of production of proinflammatory cytokines IL-1, IFNγ and inducible nitric oxide synthase. We confirmed an important beneficial role of nanofiber scaffolds in transplantation of mesenchymal stem cells. Nanofiber...
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Coaxial and emulsion nanofibers for biomedical use
Korbelová, Gabriela ; Buzgo, Matej (advisor) ; Pavlů, Barbora (referee)
The current treatment of tissue injuries deal with numerous problems, such as limited healing capacity and immune response of the body. In many cases such obstacles does not allow a full regeneration of the injured tissues and the full resumption of its original features. Controlled delivery of growth factors has considerable importance for inducing wound healing for a wide range of defects. Biocompatible and biodegradable nanofibrous carriers prepared by electrospinning represent a universal platform for the delivery of a wide range of substances in regulated manner. The goal of this thesis is to create an overview and assessment of the nanofibrous systems for controlled drug release created by electrospinning with a focus on growth factors delivery. Nanofiber carrier offers a number of advantages, they are able to consistently deliver bioactive substance into the site of injury, maintain bioactivity of the substance and stimulate the adhesion, proliferation and differentiation of target cells. Compared to the classic methods of tissue engineering using tissue grafts with the risk of their immune rejection these funkcionalized biodegradable carriers offers the possibility to create cell-free carrier systems. The work is divided into 4 chapters concerning the methods of preparation of nanofibrous...
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Coaxial and emulsion nanofibers for biomedical use
Korbelová, Gabriela ; Buzgo, Matej (advisor) ; Pavlů, Barbora (referee)
The current treatment of tissue injuries deal with numerous problems, such as limited healing capacity and immune response of the body. In many cases such obstacles does not allow a full regeneration of the injured tissues and the full resumption of its original features. Controlled delivery of growth factors has considerable importance for inducing wound healing for a wide range of defects. Biocompatible and biodegradable nanofibrous carriers prepared by electrospinning represent a universal platform for the delivery of a wide range of substances in regulated manner. The goal of this thesis is to create an overview and assessment of the nanofibrous systems for controlled drug release created by electrospinning with a focus on growth factors delivery. Nanofiber carrier offers a number of advantages, they are able to consistently deliver bioactive substance into the site of injury, maintain bioactivity of the substance and stimulate the adhesion, proliferation and differentiation of target cells. Compared to the classic methods of tissue engineering using tissue grafts with the risk of their immune rejection these funkcionalized biodegradable carriers offers the possibility to create cell-free carrier systems. The work is divided into 4 chapters concerning the methods of preparation of nanofibrous...
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Experimental models of a transfer of stem cells for therapeutic purposes
Faltýsková, Helena ; Krulová, Magdaléna (advisor) ; Indrová, Marie (referee)
Experimental models of a transfer of stem cells for therapeutic purposes Abstract Stem cell therapy currently represents a standard procedure of treating a wide variety of hereditary diseases and serious injuries. Development of the most suitable way of transfer of stem cells into the patient body remains very important question concerning this type of therapy. In our experiments we used nanofiber scaffolds for stem cell cultivation and their subsequent transfer. These nanofibers were prepared by the original needleless electrospun NanospiderTM technology. Allogeneic cornea or skin graft were transplanted from B6 mice to BALB/c mice. The grafts were covered by a nanofibrous scaffold with cultivated stem cells. Stem cells were stained by an imunofluorescent dye to enable us to monitore their migration from nanofibers into tissues and consequent distribution in the body and characterize changes of this distribution in the time. The methods of ELISA and PCR were used to confirm that mesenchymal stem cells support the production of antiinflammatory cytokines IL-4 and IL-10 and contribute to inhibition of production of proinflammatory cytokines IL-1, IFNγ and inducible nitric oxide synthase. We confirmed an important beneficial role of nanofiber scaffolds in transplantation of mesenchymal stem cells. Nanofiber...
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